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Okinawa Microscopy Workshop 2024 (Extremely Useful Materials)

Okinawa Microscopy Workshop 2024 I had the incredible opportunity to attend the microscopy workshop at the Okinawa Institute of Science and Technology . I was immersed in a wealth of microscopy knowledge and bioimage data analysis. The instructors from AIC Janelia HHMI , the Neuroscience Microscopy Core Facility at UNC , and the Image Facility at OIST were true experts, enthusiastically sharing their experiences in microscopy, bioimage analysis, and scientific integrity. They even encouraged us to share the teaching materials to help grow the bioimage community within Southeast Asia! I feel so fortunate to have been selected as a participant – I hear the competition was fierce! Workshop participants came from all across Southeast Asia (Malaysia, Cambodia, Indonesia, Singapore, Thailand, Vietnam, and the Philippines) and nearly every region of Japan! The atmosphere was dynamic, productive, and occasionally a bit stressful as we worked to complete our assignments. https://www.aicjanelia...
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The component inside the UV-Vis Spectrophotometer

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Link to the instrument booking and logbook. GENESYS™ 10S UV-Vis Spectrophotometer - ช่างเข้ามาทำ PM เลยมาสังเกตการณ์ ตัวเครื่องภายในดูบ้าง (now, we have time to observe the components inside the UV-VIS during the PM) - ถ้าดูตัวอะไหล่ข้างในจะเห็นได้ว่า มันต้องวาง position ที่เหมาะสม ไม่งั้นแล้ว light source มันเลื่อน และไม่ผ่าน sample และ detector - ดังนั้นการขนย้าย ไม่แนะนำให้วางแล้วเข็นผ่านพื้นที่มีร่อง หรือเข็นแล้วมีการกระเทือน ควรจะใช้วิธีการยก หรือใช้วิธีเคลื่อนย้ายแบบใดก็ได้ ที่ไม่มีการกระเทือนต่อ position ของตัวอะไหล่ข้างในที่เกี่ยวข้องกับระบบแสง (As you can see, each componants have to be well-aligned, it is recommended by the instrument tecnician that if the instrument is required to relocate - we need to lift the UV-VIS, rather than put it in the cart and push it through the non-smooth floor). Otherwise, those componets might be misaligned and cause the signal undetectable! - หลังเปิดฝาก็มีฝุ่น น่าจะเป็นฝุ่นที่มาจากห้องหรือตึกที่เป็นลานจอดรถ ดูจากวงสีแดง - Light source: Xenon ...
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How to properly use antiglare screen to prevent obstruction of the light path

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We have observed dim images in the top-left corner of the screen, which occurs in both fluorescence and bright-field imaging (see below image).  We believe that the antiglare screen's misalignment with the light path is causing the obstruction. This issue, which might arise when users remove or insert slides into the sample holder, has been brought to their attention.
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[Recommend to Read] Two recent papers about the bioimage core

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 https://onlinelibrary.wiley.com/doi/10.1111/jmi.13307 https://www.nature.com/articles/s41592-024-02397-1?fbclid=IwY2xjawGD68BleHRuA2FlbQIxMQABHUGehtv-4RHAs4AOA3_XPwr3elxE1aIY3Xzp9UqjeGZ7k-bL9afjHcCefg_aem_zOBSsOeIZ7RZOXrAlXFwQA#Sec5 -- I have already pointed out in the report after attending the Okinawa Microscopy Workshop 2024 :
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Lab_Instrument_Research_Center_MURA

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Research Integrity

 แนะนำให้ดู น่าจะเริ่มจริงจังขึ้นเรื่อย ๆ  Research integrity_note_DrYongPoovorawan.pdf from Tassanee Lerksuthirat Related link: https://minicore-rarc.blogspot.com/search/label/Research%20integrity
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Example of User Policy

Below are examples of publicly available user policies for developing guidelines to use with our Bioamge Facility, which I have taken care of. https://nic.botany.wisc.edu/ nic-guidelines/ https://www.med.unc.edu/ neuroscience/neuro-microscopy/ user-info/core-policies/ https://groups.oist.jp/img https://science.mahidol.ac.th/ scre/cif/?page_id=678&lang=en https://simba-mbi.com/code-of- conduct https://microscopy.duke.edu/rules Our policy
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[Recommend to Read] A Postdoctoral Training Program in Bioimage Analysis

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I cannot download the full text, but the pre-prine version is freely available. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11118354/ The tenure track is very limited, thus generating a post-doc crisis in which the majority cannot compete for the tenure track. They are looking for alternative career paths and focusing in-depth on their technical skill sets. Consequently, the group launched post-doc training on bioimage analysis, where this technique becomes more important in several industries as well as in academic environments, like core facilities. As of now, I don't see a real core facility in Thailand, only the custodians who take care of instruments but don't have in-depth knowledge of the technical part as well as data analysis related to the particular instruments.
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