Mini Core Facility

(As of Now) We have visited three vendors and collected all the images from them. Below are the links you can use to download and view the relevant files. Leica: https://webshare.leica-microsystems.com/latest/core/widefield/ (latest)  https://drive.google.com/drive/folders/1EsoXydTF66vt1vr-2seqNs-ZjjkD-It7 Zeiss: https://www.zeiss.com/microscopy/en/products/software/zeiss-zen-lite.html Olympus: https://www.olympus-lifescience.com/en/downloads/detail-iframe/?0[downloads][id]=847252030 Nikon:  https://www.microscope.healthcare.nikon.com/products/software/nis-elements/viewer

We have been using the Nikon-Ci for almost 9 years now. The mercury lamp was previously replaced at around 500 hours. This time, we used it until it reached nearly 800 hours. The signal has become dim, and dust has accumulated to the point where the heat cooler fan is starting to overload. Here is a picture showing a normal mercury lamp with no swelling at the glass ampule. Please see the second picture below for comparison. Below is the mercury lamp with a swollen glass ampule due to longer usage (pointed by skrewdriver).

- Due to the mercury lamp box spare part will be no longer supplied, thus the company recommend to replace our current light source with the LED. - LED box contains 4 LED lights, 1) 385 nm, 2) 475 nm, 3) 550 nm, 4) 621 nm as shown in the picture below. - Showing the excitation and emission profiles with current available filters that we have ( https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html ) If you would like to try it by yourself -- I would recommend to visit:  https://www.bdbiosciences.com/en-nz/resources/bd-spectrum-viewer - Testing set that we would like to try with the new LED light source - Set 1      - Nucleus – Dapi (LED 385 – filter UV-2A)      - Rad51 - Alexa fluor 555 (LED 550 – filter TexasRed)       - H2Ax -- Alexa fluor 488 (LED 475 – filter FITC) - Set 2      - Nucleus - Dapi (LED 385 – filter UV-2A)      - Rad51 - Alexa fluor...

Filter cubes Up to 5 slots, one is left in blank for the bright field Available filters in our facility; 1. UV2A (Longpass filter) 2. B2A (Longpass filter) 3. G2A (Longpass filter) 4. Texas red (Bandpass filter) 5. FITC (Bandpass filter) 6. DAPI (Bandpass filter) -- Phase contrast -- Lens: Plan Fluor 4X/0.13NA Ph1 Plan Fluor 10X/0.3NA Ph1 S Plan Fluor 20X/0.45NA (adjustable correction collar) Ph2 S Plan Fluor 40X/0.60NA (adjustable correction collar) Ph2 S Plan Apo 60X/0.7NA (adjustable correction collar) Only trained personnel will be allowed to change the filters by themselves. Check out type of filters:  https://bityl.co/C3BV Manual Nikon Ti-U (Eng-full manual) Fluorescence microscope (Ti Series) - SOP from Tassanee Lerksuthirat Link to download NIS software (only viewer - free) https://www.microscope.healthcare.nikon.com/en_AOM/products/software/nis-elements/viewer Instructional video Location Cell culture room, microbiology area Only person who has ...