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Showing posts from May, 2022

New D-LEDI from Nikon (update - 2022-08-30)

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- Due to the mercury lamp box spare part will be no longer supplied, thus the company recommend to replace our current light source with the LED. - LED box contains 4 LED lights, 1) 385 nm, 2) 475 nm, 3) 550 nm, 4) 621 nm as shown in the picture below. - Showing the excitation and emission profiles with current available filters that we have ( https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html ) If you would like to try it by yourself -- I would recommend to visit:  https://www.bdbiosciences.com/en-nz/resources/bd-spectrum-viewer - Testing set that we would like to try with the new LED light source - Set 1      - Nucleus – Dapi (LED 385 – filter UV-2A)      - Rad51 - Alexa fluor 555 (LED 550 – filter TexasRed)       - H2Ax -- Alexa fluor 488 (LED 475 – filter FITC) - Set 2      - Nucleus - Dapi (LED 385 – filter UV-2A)      - Rad51 - Alexa fluor 594 (LED 550 – filter TexasRed)      - H2Ax -- Alexa fluor 488 (LED 475 – filter FITC) -