Mini Core Facility

ZEISS LSM 910 with Lightfield 4D Unseen Speed of 80 Volumes/sec Unmatched Precision of 80 nm Cell: TK6 with GAPDH-EGFP, nucleus staining with Hoechst 33342 (1:2000) Example from our sample!! PC Specification

Prompt:  how to stitch the images in Zen software from zeiss, when the tiled images are located on different places Answer: Zeiss ZEN software is primarily designed to stitch images that were acquired as a tiled scan within the software itself, meaning the metadata contains the precise stage coordinates for each tile. This makes stitching largely automated and highly accurate. When your tiled images are located in different places (e.g., separate files, different folders, or even acquired on different systems without precise stage coordinate metadata), the stitching process becomes more manual or relies on features like ZEN Connect for alignment. Here's a breakdown of how to approach stitching in ZEN software, especially when dealing with images that weren't acquired as a single, contiguous tile scan: 1. Ideal Scenario: Images Acquired as a Tile Scan within ZEN If your "tiled images" are actually individual fields from a ZEN "Tiles" experiment (even if save...

(As of Now) We have visited three vendors and collected all the images from them. Below are the links you can use to download and view the relevant files. Leica: https://webshare.leica-microsystems.com/latest/core/widefield/ (latest)  https://drive.google.com/drive/folders/1EsoXydTF66vt1vr-2seqNs-ZjjkD-It7 Zeiss: https://www.zeiss.com/microscopy/en/products/software/zeiss-zen-lite.html Olympus: https://www.olympus-lifescience.com/en/downloads/detail-iframe/?0[downloads][id]=847252030 Nikon:  https://www.microscope.healthcare.nikon.com/products/software/nis-elements/viewer

I am not a custodian of this scientific instrument but I have to use it. I asked the custodian to make video in order for the users to self-learn and remind. To download the software; https://www.zeiss.com/microscopy/en/products/software/zeiss-zen-lite.html Manual The imageJ can open the .czi file. How to use ZEN lite (require dongle to analyze the full mode) Location: cell culture II Booking: Log-book

Model: Axio Imager 2 (Fully Motorized) - has started the mission on 26 September 2024 White light source: LED Illuminator microLED 2 1.7.1 423053-9072-000 Fluorescence light source: LED Colibri 7 , Type R[G/Y]B-UV - 423052-9730-000 UV (385/30 nm - 370-400 nm)   for excitation of DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue and similar dyes Blue (469/38 nm - 450-488 nm)   for excitation of FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC/ Fluorescein, Kaede (green /red), PerCP, YoYo-1, FuraRed and similar dyes Green (555/30 nm - 540-570 nm)   for excitation of TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange and similar dyes Yellow (590/27 nm - 576-603 nm)   for excitation of MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, R...