Upright Fluorescence Microscope (update 13 Dec 2024)
Model: Axio Imager 2 (Fully Motorized) - has started the mission on 26 September 2024
White light source: LED
Illuminator microLED 2 1.7.1 423053-9072-000
Fluorescence light source: LED
Colibri 7, Type R[G/Y]B-UV - 423052-9730-000
White light source: LED
Illuminator microLED 2 1.7.1 423053-9072-000
Fluorescence light source: LED
Colibri 7, Type R[G/Y]B-UV - 423052-9730-000
UV (385/30 nm - 370-400 nm) for excitation of DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue and similar dyes
Blue (469/38 nm - 450-488 nm) for excitation of FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC/ Fluorescein, Kaede (green /red), PerCP, YoYo-1, FuraRed and similar dyes
Green (555/30 nm - 540-570 nm) for excitation of TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange and similar dyes
Yellow (590/27 nm - 576-603 nm) for excitation of MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR and similar dyes
Red (631/33 nm - 614-647 nm) for excitation of Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N and similar dyes
Available filters
1. Filter Set 02 DAPI 488002-9901-000
G 365 FT 395 LP 397
2. Filter set 44 FITC 000000-1114-459
EX BP 475/40, BS FT 500, EM BP 530/50
3. Cy 3 Chroma 39004
Blue (469/38 nm - 450-488 nm) for excitation of FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC/ Fluorescein, Kaede (green /red), PerCP, YoYo-1, FuraRed and similar dyes
Green (555/30 nm - 540-570 nm) for excitation of TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange and similar dyes
Yellow (590/27 nm - 576-603 nm) for excitation of MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR and similar dyes
Red (631/33 nm - 614-647 nm) for excitation of Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N and similar dyes
Available filters
1. Filter Set 02 DAPI 488002-9901-000
G 365 FT 395 LP 397
2. Filter set 44 FITC 000000-1114-459
EX BP 475/40, BS FT 500, EM BP 530/50
3. Cy 3 Chroma 39004
EX AT540/25x, BS AT565DC, EM AT605/55m
EX BP 640/30, BS FT 660, EM BP 690/50
5. 90 HE DAPI/ GFP/ Cy3/ Cy5 489090-9110-000
5. 90 HE DAPI/ GFP/ Cy3/ Cy5 489090-9110-000
EX BP 631/33
Objective Lens:
N-Achroplan 5x/0.15 M27 (FWD=12.0mm)
N-Achroplan 10x/0.25 M27 (FWD=6.5mm)
N-Achroplan 20x/0.45 M27 (FWD=0.63mm)
EC Plan-Neofluar 40x/0.75 M27 (FWD=0.71mm), incl. Cover glasses, high performance
EC Plan-Neofluar 100x/1.30 Oil M27 (FWD=0.20mm), incl. Immersol 518 F
Camera: 2 cameras
Color - ZEISS Axiocam 305 color
Sensor Model: Sony IMX 264 Exmor Pregius, CMOS
Sensor Pixel Count: 5 Megapixel: 2,464 (H) x 2,056 (V)
Pixel Size: 3.45 µm x 3.45 µm
Sensor Size: 8.5 mm x 7.1 mm; image diagonal 11.1 mm, equivalent to ⅔" sensor format
Spectral Range: Approx. 400 nm - 720 nm, coated Hoya C5000 IR Cut Filter; RGB Bayer color filter mask
Mono - ZEISS Axiocam 807 mono
Sensor Type Sony CMOS image sensor, Global Shutter architecture
Beam splitter: QBS 405+493+575+653
Emission: QBP 425/30+514/30+592/25+709/100
Objective Lens:
N-Achroplan 5x/0.15 M27 (FWD=12.0mm)
N-Achroplan 10x/0.25 M27 (FWD=6.5mm)
N-Achroplan 20x/0.45 M27 (FWD=0.63mm)
EC Plan-Neofluar 40x/0.75 M27 (FWD=0.71mm), incl. Cover glasses, high performance
EC Plan-Neofluar 100x/1.30 Oil M27 (FWD=0.20mm), incl. Immersol 518 F
Camera: 2 cameras
Color - ZEISS Axiocam 305 color
Sensor Model: Sony IMX 264 Exmor Pregius, CMOS
Sensor Pixel Count: 5 Megapixel: 2,464 (H) x 2,056 (V)
Pixel Size: 3.45 µm x 3.45 µm
Sensor Size: 8.5 mm x 7.1 mm; image diagonal 11.1 mm, equivalent to ⅔" sensor format
Spectral Range: Approx. 400 nm - 720 nm, coated Hoya C5000 IR Cut Filter; RGB Bayer color filter mask
Mono - ZEISS Axiocam 807 mono
Sensor Type Sony CMOS image sensor, Global Shutter architecture
Mono and color versions
Sensor Size Image Diagonal 17.6 mm, equivalent to 1.1" Sensor Format
Image Field (14.5 mm x 9.9 mm)
Sensor Pixel Count 3216 (H) x 2208 (V) = 7.1 Megapixel
HW Subsampling 2x 1608 (H) x 1104 (V) = 1.8 Megapixel, high speed full view mode
Pixel Size 4.5 µm x 4.5 µm
Sensor Size Image Diagonal 17.6 mm, equivalent to 1.1" Sensor Format
Image Field (14.5 mm x 9.9 mm)
Sensor Pixel Count 3216 (H) x 2208 (V) = 7.1 Megapixel
HW Subsampling 2x 1608 (H) x 1104 (V) = 1.8 Megapixel, high speed full view mode
Pixel Size 4.5 µm x 4.5 µm
Program: ZEN 3.10 (ZEN Pro)
Only a person who has a Gmail account can get access to the calendar.
More information on specifications on MU-REX portal
Operating manual
Operating manual + ZEN lite
Check crosstalk and Dye compatibility
Manual (Full) + Regulation
Custodian
1.Tassanee Lerksuthirat
2.Chalisa Jaturapaktrarak
3.Pawarit Innachai
Location
Important on objective lens!!!
This is a very good video describing the damage to the objective lens!
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Decommission 24 Sep 2024
Nikon upright fluorescence microscope - bye-bye 👋👋👋
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