Upright Fluorescence Microscope (update 14 Apr 2026)

Model: Axio Imager 2 (Fully Motorized) - has started the mission on 26 September 2024

White light source: LED
Illuminator microLED 2 1.7.1 423053-9072-000

Fluorescence light source: LED
Colibri 7, Type R[G/Y]B-UV - 423052-9730-000

UV (385/30 nm - 370-400 nm) for excitation of DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue and similar dyes
Blue (469/38 nm - 450-488 nm) for excitation of FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC/ Fluorescein, Kaede (green /red), PerCP, YoYo-1, FuraRed and similar dyes
Green (555/30 nm - 540-570 nm) for excitation of TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange and similar dyes
Yellow (590/27 nm - 576-603 nm) for excitation of MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR and similar dyes
Red (631/33 nm - 614-647 nm) for excitation of Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N and similar dyes

Available filters
1. Filterset 96 HE BFP

Item no: 489096-9100-000:

EX BP 390/40, BS FT 420, EM BP 450/40

https://www.micro-shop.zeiss.com/en/pl/fluorescenceAssistant/filtersets/489096-9100-000

2. Alexa flour 488 zone:

Semrock

475/35 nm BrightLine® single-band bandpass filter

https://www.idex-hs.com/store/product-detail/ff01_475_35_25/fl-004539?search=true

495 nm edge BrightLine® single-edge standard epi-fluorescence dichroic beamsplitter

https://www.idex-hs.com/store/product-detail/ff495_di03_25x36/fl-007145?search=true

515/30 nm BrightLine® single-band bandpass filter

https://www.idex-hs.com/store/product-detail/ff01_515_30_25/fl-004382?search=true

3. Cy 3 Chroma 39004

EX AT540/25x, BS AT565DC, EM AT605/55m

4. 63 HE mRFP 489063-0000-000

EX BP 640/30, BS FT 660, EM BP 690/50
5. FL Filter Set 50 Cy5, EX BP640/30 S free

Item no: 488050-9901-000

EX BP 640/30, BS FT 660, EM BP 690/50

https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488050-9901-000

Objective Lens:
N-Achroplan 5x/0.15 M27 (FWD=12.0mm)
N-Achroplan 10x/0.25 M27 (FWD=6.5mm)
N-Achroplan 20x/0.45 M27 (FWD=0.63mm)
EC Plan-Neofluar 40x/0.75 M27 (FWD=0.71mm), incl. Cover glasses, high performance
EC Plan-Neofluar 100x/1.30 Oil M27 (FWD=0.20mm), incl. Immersol 518 F

Camera: 2 cameras
Color - ZEISS Axiocam 305 color
Sensor Model: Sony IMX 264 Exmor Pregius, CMOS
Sensor Pixel Count: 5 Megapixel: 2,464 (H) x 2,056 (V)
Pixel Size: 3.45 µm x 3.45 µm
Sensor Size: 8.5 mm x 7.1 mm; image diagonal 11.1 mm, equivalent to ⅔" sensor format
Spectral Range: Approx. 400 nm - 720 nm, coated Hoya C5000 IR Cut Filter; RGB Bayer color filter mask

Mono - ZEISS Axiocam 807 mono
Sensor Type Sony CMOS image sensor, Global Shutter architecture

Mono and color versions
Sensor Size Image Diagonal 17.6 mm, equivalent to 1.1" Sensor Format
Image Field (14.5 mm x 9.9 mm)
Sensor Pixel Count 3216 (H) x 2208 (V) = 7.1 Megapixel
HW Subsampling 2x 1608 (H) x 1104 (V) = 1.8 Megapixel, high speed full view mode
Pixel Size 4.5 µm x 4.5 µm

Program: ZEN 3.10 (ZEN Pro)

Only a person who has a Gmail account can get access to the calendar.

More information on specifications on MU-REX portal

https://mahidol.elsevierpure.com/en/equipments/motorized-upright-fluorescence-microscope

Operating manual

https://www.rama.mahidol.ac.th/ramacloud/s/QuEavNgsKGJSrMQ

Operating manual + ZEN lite

https://drive.google.com/drive/folders/1JW6ljt3weToWRRFTTI4jhhm0iO-93fWt

Check crosstalk and Dye compatibility

https://www.fpbase.org/microscope/R3hqcsiTCe7D5rSueT8UsZ/ (new-use since 25Mar2026)

https://www.fpbase.org/microscope/DQNDPMbn4QfycdqkLhPizE/ (old)

Manual (Full) + Regulation