Upright Fluorescence Microscope (update 10-Oct-2024)

Model: Axio Imager 2 (Fully Motorized) - has started the mission on 26 September 2024

White light source: LED
Illuminator microLED

Fluorescence light source: LED
Colibri 7, Type R[G/Y]B-UV
UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes
Blue (475nm) for excitation of eGFP, Fluo4, FITC and similar
Green (555nm) for excitation of Cy3, TRITC, DsRed and similar dyes
Yellow (590nm) for excitation of mCherry, Alex 568, mPlum and similar dyes
Red (630nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes

Available filters
1. Filter set 49 DAPI shift free (E)
EX G 365, BS FT 395, EM BP 445/50
2. Filter set 44 FITC special, d=25 shift free (E)
EX BP 475/40, BS FT 500, EM BP 530/50
3. Filter set 45 HQ TexasRed, d=25 shift free (E)
EX BP 560/40, BS FT 585, EM BP 630/75
4. Filter set 50 Cy 5 shift free (E)
EX BP 640/30, BS FT 660, EM BP 690/50
5. Filter set 43 Cy 3, d=25 shift free (E)
EX BP 545/25, BS FT 570, EM BP 605/70

Objective Lens:
N-Achroplan 5x/0.15 M27 (FWD=12.0mm)
N-Achroplan 10x/0.25 M27 (FWD=6.5mm) 
N-Achroplan 20x/0.45 M27 (FWD=0.63mm)
EC Plan-Neofluar 40x/0.75 M27 (FWD=0.71mm), incl. Cover glasses, high performance
EC Plan-Neofluar 100x/1.30 Oil M27 (FWD=0.20mm), incl. Immersol 518 F

Camera: 2 cameras
Color - ZEISS Axiocam 305 color
Sensor Model: Sony IMX 264 Exmor Pregius, CMOS
Sensor Pixel Count: 5 Megapixel: 2,464 (H) x 2,056 (V)
Pixel Size: 3.45 µm x 3.45 µm
Sensor Size: 8.5 mm x 7.1 mm; image diagonal 11.1 mm, equivalent to ⅔" sensor format
Spectral Range: Approx. 400 nm - 720 nm, coated Hoya C5000 IR Cut Filter; RGB Bayer color filter mask

Mono - ZEISS Axiocam 807 mono
Sensor Type Sony CMOS image sensor, Global Shutter architecture
Mono and color versions
Sensor Size Image Diagonal 17.6 mm, equivalent to 1.1" Sensor Format
Image Field (14.5 mm x 9.9 mm)
Sensor Pixel Count 3216 (H) x 2208 (V) = 7.1 Megapixel
HW Subsampling 2x 1608 (H) x 1104 (V) = 1.8 Megapixel, high speed full view mode
Pixel Size 4.5 µm x 4.5 µm

Program: ZEN 3.10 (ZEN Pro)

More information on specifications on MU-REX portal




Location














Important on objective lens!!!
This is a very good video describing the damage to the objective lens!

-------
Decommission 24 Sep 2024
Nikon upright fluorescence microscope - bye-bye 👋👋👋

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