Mini Core Facility

Prompt:  how to stitch the images in Zen software from zeiss, when the tiled images are located on different places Answer: Zeiss ZEN software is primarily designed to stitch images that were acquired as a tiled scan within the software itself, meaning the metadata contains the precise stage coordinates for each tile. This makes stitching largely automated and highly accurate. When your tiled images are located in different places (e.g., separate files, different folders, or even acquired on different systems without precise stage coordinate metadata), the stitching process becomes more manual or relies on features like ZEN Connect for alignment. Here's a breakdown of how to approach stitching in ZEN software, especially when dealing with images that weren't acquired as a single, contiguous tile scan: 1. Ideal Scenario: Images Acquired as a Tile Scan within ZEN If your "tiled images" are actually individual fields from a ZEN "Tiles" experiment (even if save...

A very good suggested guideline article for publishing microscope image data: check out Nature Method. Community-developed checklists for publishing images and image analyses https://www.nature.com/articles/s41592-023-01987-9 As of now, our facility still lacks "Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy (QUAREP-LiMi)" , and I don't see any support from any leader at any level so far --- yet, the University would like us to have good quality research. As I have asked the distributors (4 distributors -- Nikon, Leica, Olympus, and ZEISS), none of them have known this issue, and the specialists lack the program to initiate it. This is because it has costs, and they don't know whether each central facility in each University would like to have this. If no customer, it is worthless for them to initiate this service, thus the core must do it by itself, and it requires skilled personnel and financial support. Old blogs related to ...

What kind of image manipulation that they have conclude? Category I is defined as, "Figures containing two or more identical panels, either within the same figure or between different figures within the same paper, purporting to represent different experimental conditions." Category II ".included microscopic or blot images with a clear region of overlap, where one image had been shifted, rotated, or reversed with respect to the other." Category III ".consisted of images that were altered with complete or partial duplication of lanes, bands, groups of cells.   sometimes with rotation or reversal with respect to each other, within the same image panel or between panels or figures. " Check out these two webs for image processing; https://www.imagetwin.io/ github.com/GuidoBartoli/sherloq Original article; https://www.helmholtz-helena.de/fileadmin/Scientific_training/Literature/Wjst_2020_231_Kopie.pdf 10.1165/rcmb.2020-0419LE

For Ramathibodi Hospital staff 1.Hospital Annual Report https://med.mahidol.ac.th/th/annual_reports 2. Research fund and data analysis https://www.rama.mahidol.ac.th/research/fundsandanalysis/th 3. Online reserve meeting room Research Center RAMA http://10.6.155.94/RAI/index.php/roombooking/index/1 3.1. Rule for meeting room reservation Click here!!! The system is not good enough - they don't have the online cancel. 4.  Submitting the projects to Research Center RAMA (new version) https://www3.ra.mahidol.ac.th/ERequest/ All the data from the old version cannot be transferred. 5.Safety training/self-educate @ Research Center RAMA https://sites.google.com/site/rclabsafety/ 5.1.Registered microorganisms https://goo.gl/forms/6sVxlxJGJhpyw7QN2 6. RC-related forms and documents \\10.6.155.100\f\ แบบฟอร์มต่างๆสำนักงานวิจัย (via LAN) http://wss9/sites/hospital/research  (through RAMA only intranet) 7.RC research center https://med.mah...