Mini Core Facility

Tools 1.Inverted fluorescence microscope https://minicore-rarc.blogspot.com/2018/05/inverted-fluorescence-microscope.html 2.Upright fluorescence microscope https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html 3.UV-Vis spectrophotometer https://minicore-rarc.blogspot.com/2019/02/genesys-10s-uv-vis-spectrophotometer.html 4.UVP Hybridization https://minicore-rarc.blogspot.com/2020/02/uvp-hybridization.html 5.Boekel Hybridization https://minicore-rarc.blogspot.com/2020/02/wi-mi-09boekel-big-shot-iii.html 6.Electroporation https://minicore-rarc.blogspot.com/2020/10/electroporation.html Online calendar for all instruments that I have been assigned as custodian. Click How to book the instrument Guidelines to determine the authorship for the people who work in the core facility Click

To compare the rate - Update in 2019; Ramathibodi Hospital (RC and Patho) Patho Ramathibodi Hospital TU-SAT

For Ramathibodi Hospital staff 1.Hospital Annual Report https://med.mahidol.ac.th/th/annual_reports 2. Research fund and data analysis https://www.rama.mahidol.ac.th/research/fundsandanalysis/th 3. Online reserve meeting room Research Center RAMA http://10.6.155.94/RAI/index.php/roombooking/index/1 3.1. Rule for meeting room reservation Click here!!! The system is not good enough - they don't have the online cancel. 4.  Submitting the projects to Research Center RAMA (new version) https://www3.ra.mahidol.ac.th/ERequest/ All the data from the old version cannot be transferred. 5.Safety training/self-educate @ Research Center RAMA https://sites.google.com/site/rclabsafety/ 5.1.Registered microorganisms https://goo.gl/forms/6sVxlxJGJhpyw7QN2 6. RC-related forms and documents \\10.6.155.100\f\ แบบฟอร์มต่างๆสำนักงานวิจัย (via LAN) http://wss9/sites/hospital/research  (through RAMA only intranet) 7.RC research center https://med.mah...

Filter cubes Up to 5 slots, one is left in blank for the bright field Available filters in our facility; 1. UV2A (Longpass filter) 2. B2A (Longpass filter) 3. G2A (Longpass filter) 4. Texas red (Bandpass filter) 5. FITC (Bandpass filter) 6. DAPI (Bandpass filter) -- Phase contrast -- Lens: Plan Fluor 4X/0.13NA Ph1 Plan Fluor 10X/0.3NA Ph1 S Plan Fluor 20X/0.45NA (adjustable correction collar) Ph2 S Plan Fluor 40X/0.60NA (adjustable correction collar) Ph2 S Plan Apo 60X/0.7NA (adjustable correction collar) Only trained personnel will be allowed to change the filters by themselves. Check out type of filters:  https://bityl.co/C3BV Manual Nikon Ti-U (Eng-full manual) Fluorescence microscope (Ti Series) - SOP from Tassanee Lerksuthirat Link to download NIS software (only viewer - free) https://www.microscope.healthcare.nikon.com/en_AOM/products/software/nis-elements/viewer Instructional video Location Cell culture room, microbiology area Only person who has ...

Model: Axio Imager 2 (Fully Motorized) - has started the mission on 26 September 2024 White light source: LED Illuminator microLED 2 1.7.1 423053-9072-000 Fluorescence light source: LED Colibri 7 , Type R[G/Y]B-UV - 423052-9730-000 UV (385/30 nm - 370-400 nm)   for excitation of DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue and similar dyes Blue (469/38 nm - 450-488 nm)   for excitation of FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC/ Fluorescein, Kaede (green /red), PerCP, YoYo-1, FuraRed and similar dyes Green (555/30 nm - 540-570 nm)   for excitation of TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange and similar dyes Yellow (590/27 nm - 576-603 nm)   for excitation of MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, R...