Inverted Fluorescence Microscope (update: 2024-06-01)

Filter cubes
Up to 5 slots, one is left in blank for the bright field

Available filters in our facility;
1. UV2A (Longpass filter)
2. B2A (Longpass filter)
3. G2A (Longpass filter)
4. Texas red (Bandpass filter)
5. FITC (Bandpass filter)
6. DAPI (Bandpass filter)
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Phase contrast
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Lens:
Plan Fluor 4X/0.13NA
Ph1 Plan Fluor 10X/0.3NA
Ph1 S Plan Fluor 20X/0.45NA (adjustable correction collar)
Ph2 S Plan Fluor 40X/0.60NA (adjustable correction collar)
Ph2 S Plan Apo 60X/0.7NA (adjustable correction collar)

Only trained personnel will be allowed to change the filters by themselves.
Check out type of filters: https://bityl.co/C3BV

 Manual
Nikon Ti-U (Eng-full manual)


Link to download NIS software (only viewer - free)

Instructional video


Location
Cell culture room, microbiology area


Only person who has a gmail account can get access to the calendar.




Important on objective lens!!!

This is a very good video describing the damage to the objective lens!
 


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Quick links for all tools

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Tools 1.Inverted fluorescence microscope https://minicore-rarc.blogspot.com/2018/05/inverted-fluorescence-microscope.html 2.Upright fluorescence microscope https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html 3.UV-Vis spectrophotometer https://minicore-rarc.blogspot.com/2019/02/genesys-10s-uv-vis-spectrophotometer.html 4.UVP Hybridization https://minicore-rarc.blogspot.com/2020/02/uvp-hybridization.html 5.Boekel Hybridization https://minicore-rarc.blogspot.com/2020/02/wi-mi-09boekel-big-shot-iii.html 6.Electroporation https://minicore-rarc.blogspot.com/2020/10/electroporation.html Online calendar for all instruments that I have been assigned as custodian. Click How to book the instrument Guidelines to determine the authorship for the people who work in the core facility Click RC Instrument list (yearly check)
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