New D-LEDI from Nikon (update - 2022-08-30)

- Due to the mercury lamp box spare part will be no longer supplied, thus the company recommend to replace our current light source with the LED.

- LED box contains 4 LED lights, 1) 385 nm, 2) 475 nm, 3) 550 nm, 4) 621 nm as shown in the picture below.


- Showing the excitation and emission profiles with current available filters that we have (https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html)


If you would like to try it by yourself -- I would recommend to visit: https://www.bdbiosciences.com/en-nz/resources/bd-spectrum-viewer

- Testing set that we would like to try with the new LED light source

- Set 1
    - Nucleus – Dapi (LED 385 – filter UV-2A)
    - Rad51 - Alexa fluor 555 (LED 550 – filter TexasRed) 
    - H2Ax -- Alexa fluor 488 (LED 475 – filter FITC)
- Set 2
    - Nucleus - Dapi (LED 385 – filter UV-2A)
    - Rad51 - Alexa fluor 594 (LED 550 – filter TexasRed)
    - H2Ax -- Alexa fluor 488 (LED 475 – filter FITC)
- Set 3
    - Nucleus - Dapi (LED 385 – filter UV-2A)
    - Rad51 - Alexa fluor 568 (LED 550 – filter TexasRed)
    - H2Ax -- Alexa fluor 488 (LED 475 – filter FITC)
- Set 4
    - Nucleus - Dapi (LED 385 – filter UV-2A)
    - Rad51 - Alexa fluor 647 (LED621 – filter Cy5)
    - H2Ax -- Alexa fluor 488 (LED 475 – filter FITC)
- Set 5
    - Nucleus - Dapi (LED 385 – filter UV-2A)
    - Rad51 - Alexa fluor 488 (LED 475 – filter FITC)
    - H2Ax -- Alexa fluor 594 (LED 550 – filter TexasRed)

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I assume the best combination would be:
Dapi+AF488+AF568+AF647

Results (update 2022-08-30)




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Another website -- contain many fluorophores
https://www.aatbio.com/fluorescence-excitation-emission-spectrum-graph-viewer




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