New D-LEDI from Nikon (update

- Due to the mercury lamp box spare part will be no longer supplied, thus the company recommend to replace our current light source with the LED.

- LED box contains 4 LED lights, 1) 385 nm, 2) 475 nm, 3) 550 nm, 4) 621 nm as shown in the picture below.

- Showing the excitation and emission profiles with current available filters that we have (https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html)

If you would like to try it by yourself -- I would recommend to visit: https://www.bdbiosciences.com/en-nz/resources/bd-spectrum-viewer

- Testing set that we would like to try with the new LED light source

- Set 1

- Nucleus – Dapi (LED 385 – filter UV-2A)

- Rad51 - Alexa fluor 555 (LED 550 – filter TexasRed)

- H2Ax -- Alexa fluor 488 (LED 475 – filter FITC)

- Set 2

- Nucleus - Dapi (LED 385 – filter UV-2A)

- Rad51 - Alexa fluor 594 (LED 550 – filter TexasRed)

- H2Ax -- Alexa fluor 488 (LED 475 – filter FITC)

- Set 3

- Nucleus - Dapi (LED 385 – filter UV-2A)

- Rad51 - Alexa fluor 568 (LED 550 – filter TexasRed)

- H2Ax -- Alexa fluor 488 (LED 475 – filter FITC)

- Set 4

- Nucleus - Dapi (LED 385 – filter UV-2A)

- Rad51 - Alexa fluor 647 (LED621 – filter Cy5)

- H2Ax -- Alexa fluor 488 (LED 475 – filter FITC)

- Set 5

- Nucleus - Dapi (LED 385 – filter UV-2A)

- Rad51 - Alexa fluor 488 (LED 475 – filter FITC)

- H2Ax -- Alexa fluor 594 (LED 550 – filter TexasRed)

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I assume the best combination would be:

Dapi+AF488+AF568+AF647

Results (update 2022-08-30)

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Another website -- contain many fluorophores

Mini Core Facility