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Mini Core Facility

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Boekel Big SHOT III

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February 02, 2020

Hybridization Oven (Boekel) SOP from Tassanee Lerksuthirat

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Link to how to create mahidol.edu (google for education)

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Link to the log-book

boekel Hybridization

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How to access the full text using the EZProxy Extension in Chrome (for MU staff)

October 06, 2022

Upright Fluorescence Microscope (update 13 Dec 2024)

May 28, 2018

Model: Axio Imager 2 (Fully Motorized) - has started the mission on 26 September 2024 White light source: LED Illuminator microLED 2 1.7.1 423053-9072-000 Fluorescence light source: LED Colibri 7 , Type R[G/Y]B-UV - 423052-9730-000 UV (385/30 nm - 370-400 nm) for excitation of DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue and similar dyes Blue (469/38 nm - 450-488 nm) for excitation of FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC/ Fluorescein, Kaede (green /red), PerCP, YoYo-1, FuraRed and similar dyes Green (555/30 nm - 540-570 nm) for excitation of TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange and similar dyes Yellow (590/27 nm - 576-603 nm) for excitation of MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, R...

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October 07, 2018

Tools 1.Inverted fluorescence microscope https://minicore-rarc.blogspot.com/2018/05/inverted-fluorescence-microscope.html 2.Upright fluorescence microscope https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html 3.UV-Vis spectrophotometer https://minicore-rarc.blogspot.com/2019/02/genesys-10s-uv-vis-spectrophotometer.html 4.UVP Hybridization https://minicore-rarc.blogspot.com/2020/02/uvp-hybridization.html 5.Boekel Hybridization https://minicore-rarc.blogspot.com/2020/02/wi-mi-09boekel-big-shot-iii.html 6.Electroporation https://minicore-rarc.blogspot.com/2020/10/electroporation.html Online calendar for all instruments that I have been assigned as custodian. Click How to book the instrument Guidelines to determine the authorship for the people who work in the core facility Click RC Instrument list (yearly check)