Basic Molecular Biology Techniques

In this blog, I will compile online tutorials that demonstrate how to use basic molecular instruments correctly. As I have observed, some students/staff do not enroll in the instrumental course as a prerequisite, and as a result, they use some instruments without proper care, resulting in the instrument being damaged (wasting time and money on situations which could be prevented if they are knowledgeable).

Micropipette

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Upright Fluorescence Microscope (update 10-Oct-2024)

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Model: Axio Imager 2 (Fully Motorized) - has started the mission on 26 September 2024 White light source: LED Illuminator microLED Fluorescence light source: LED Colibri 7, Type R[G/Y]B-UV UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes Blue (475nm) for excitation of eGFP, Fluo4, FITC and similar Green (555nm) for excitation of Cy3, TRITC, DsRed and similar dyes Yellow (590nm) for excitation of mCherry, Alex 568, mPlum and similar dyes Red (630nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes Available filters 1. Filter set 49 DAPI shift free (E) EX G 365, BS FT 395, EM BP 445/50 2. Filter set 44 FITC special, d=25 shift free (E) EX BP 475/40, BS FT 500, EM BP 530/50 3. Filter set 45 HQ TexasRed, d=25 shift free (E) EX BP 560/40, BS FT 585, EM BP 630/75 4. Filter set 50 Cy 5 shift free (E) EX BP 640/30, BS FT 660, EM BP 690/50 5. Filter set 43 Cy 3, d=25 shift free (E) EX BP 545/25, BS FT 570, EM BP 605/70 Objective Lens: N-Achroplan 5x/0.15
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Quick links for all tools

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Tools 1.Inverted fluorescence microscope https://minicore-rarc.blogspot.com/2018/05/inverted-fluorescence-microscope.html 2.Upright fluorescence microscope https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html 3.UV-Vis spectrophotometer https://minicore-rarc.blogspot.com/2019/02/genesys-10s-uv-vis-spectrophotometer.html 4.UVP Hybridization https://minicore-rarc.blogspot.com/2020/02/uvp-hybridization.html 5.Boekel Hybridization https://minicore-rarc.blogspot.com/2020/02/wi-mi-09boekel-big-shot-iii.html 6.Electroporation https://minicore-rarc.blogspot.com/2020/10/electroporation.html Online calendar for all instruments that I have been assigned as custodian. Click How to book the instrument Guidelines to determine the authorship for the people who work in the core facility Click RC Instrument list (yearly check)
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