Update on the microscope condition

We have just learnt from the Nikon technician that our fiber optic has been damaged. This causes a shadow background while visualizing the sample with a mercury lamp (attached herewith), not a bright field. Besides, all the lenses have been contaminated by the immersion oil. This means that there might be some spilled over while rotating the objective lens. Please make sure to clean "all the objective lenses" properly after finishing your work. Learn from my deposited video if you are not sure how to properly clean. The 60X objective lens of the upright microscope does not work properly anymore and I decided not to replace it with a new one since it is extremely expensive. I decided to switch the optic fiber between inverted and upright. Thus, the shadow will instead appear in the inverted microscope in the cell culture-micro room. We need to place an order for the new optic fiber and I do not know when it will be approved (cost perhaps >80,000 baht). The FITC filter has to be replaced for both microscopes (~60,000 baht each) and the mercury lamp has to be replaced for the inverted microscope. To prevent the optic fiber being damaged again, I have put the marks just to fix the position of both the microscope and the mercury light source (attached pictures herewith). "Do not push the microscope away from the mark", otherwise the optic fiber (black wire connected at the back of the microscope) will be damaged. When covering the microscope with cloth, "please be aware not to harsh the fiber optic cord". My recommendation is "Please do not cover the fiber optic cord", just leave it uncovered (attached image herewith).


Images from damaged fiber optics




Be aware of the position!!



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