Mini Core Facility

A very good suggested guideline article for publishing microscope image data: check out Nature Method. Community-developed checklists for publishing images and image analyses https://www.nature.com/articles/s41592-023-01987-9 As of now, our facility still lacks "Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy (QUAREP-LiMi)" , and I don't see any support from any leader at any level so far --- yet, the University would like us to have good quality research. As I have asked the distributors (4 distributors -- Nikon, Leica, Olympus, and ZEISS), none of them have known this issue, and the specialists lack the program to initiate it. This is because it has costs, and they don't know whether each central facility in each University would like to have this. If no customer, it is worthless for them to initiate this service, thus the core must do it by itself, and it requires skilled personnel and financial support. Old blogs related to ...

This computer is attached with upright microscope. As of now (202-19-09), the RAM is 4GB, it crashes several time during taking the images after the OS has been upgraded to Windows 11. Ref:  https://dl.dell.com/topicspdf/vostro-3670-desktop_owners-manual3_en-us.pdf Expired 11 JAN 2020

 This information is used for selecting the sensor chip of the camera in the microscope. Type Designation Approximate Sensor Diagonal (mm) 1" 16.0 1/1.2" 13.4 1/1.7" 11.0 1/1.8" 10.8 2/3" 11.0 1/2.3" 7.7 1/2.5" 7.2 1/2.8" 6.4 1/3" 6.0 1/3.2" 5.7 1/4" 4.5 1/5" 3.6

Meet president (2023-06-12) Adapt to agile system A cademic G lobal+experiential I ndustry relevant – connect to real working industry L ifelong learning Education is short but we live longer, thus, we need to relearn, esp. alumni  E ntrepreneurial mindset MU wants to be top 100 within 2573 Curriculum has to be updated and more globalization แผน 20 ปี บอกเป้าหมายกับหนทาง แต่เป็นแบบหยาบ ๆ แต่ก็จะมีการปรับเป็นระยะ ๆ เพื่อให้มันสอดคล้องกับสถานการณ์ ถ้าจะเปิดเป็น service ก็ต้องมีการ accredit มีระบบการตรวจสอบทางคุณภาพ Flagship เป็นโครงการปักธงเพื่อที่จะเดินทางเข้าสู่เป้าหมาย เป็นสิ่งที่ต้องรายการกับ คกก สภามหาวิทยาลัย แยกมหาวิทยาลัยออกจาก รพ. ก่อน เพราะว่าคนติดภาพจำว่า มม เกี่ยวเนื่องกับ รพ. แต่จริง ๆ แล้วไม่ใช่ เพราะยังมี คณะอื่น ๆ อีก ดังนั้น มหาวิทยาลัยต้องโฆษณาตัวเองว่ามีความสำคัญต่อสังคมอย่างไรบ้าง การ drive พันธกิจ โดยส่วนใหญ่จะใช้เงินจากส่วนงาน ดังนั้นต้องใช้อย่างประหยัด และคุ้ม โครงการที่มี ที่จะตั้งขึ้นก็ต้องคุ้มทุน ดีไม่ดี มีแนวโน้มว่า มหาวิทยาลัย กับ รพ. อาจจะไม่มีการสนับสนุน...

 Practical Guide to Storage of Large Amounts of Microscopy Data 10.1017/S1551929520001091 Factors to Consider When Selecting Student Microscopes for Schools and Universities 10.1017/S155192951800144X How to Get Better Fluorescence Images with Your Widefield Microscope: A Methodology Review 10.1017/S155192952000156X Practical Guide to Choosing a Microscope Camera 10.1017/S155192951700061X An Image is Everything: A Tutorial on Choosing and Using the Components of a Dynamic Data Capture System 10.1017/S1551929520001558 Scientific Ethics: What Are Our Responsibilities As Facility Managers? M&M 2001 Expert's Session on Core Facility Management 10.1017/S1551929500057795 Practical Guide to Storage of Large Amounts of Microscopy Data 10.1017/S1551929520001091

Calibrating excitation light fluxes for quantitative light microscopy in cell biology https://www.nature.com/articles/nprot.2008.180 Recent advances in the standardization of fluorescence microscopy for quantitative image analysis https://link-springer-com/article/10.1007/s12551-021-00871-0#ref-CR15 Quality assessment in light microscopy for routine use through simple tools and robust metrics https://rupress.org/jcb/article-standard/221/11/e202107093/213512/Quality-assessment-in-light-microscopy-for-routine Reproducibility in light microscopy: Maintenance, standards and SOPs https://www-sciencedirect-com/science/article/pii/S1357272517301449?via%3Dihub#tbl0005 Accuracy and precision in quantitative fluorescence microscopy https://rupress.org/jcb/article/185/7/1135/35453/Accuracy-and-precision-in-quantitative A guide to accurate reporting in digital image acquisition – can anyone replicate your microscopy data? https://journals-biologists-com/jcs/article/134/6/jcs254144/237887/A-guide-t...

(As of Now) We have visited three vendors and collected all the images from them. Below are the links you can use to download and view the relevant files. Leica: https://webshare.leica-microsystems.com/latest/core/widefield/ (latest)  https://drive.google.com/drive/folders/1EsoXydTF66vt1vr-2seqNs-ZjjkD-It7 Zeiss: https://www.zeiss.com/microscopy/en/products/software/zeiss-zen-lite.html Olympus: https://www.olympus-lifescience.com/en/downloads/detail-iframe/?0[downloads][id]=847252030 Nikon:  https://www.microscope.healthcare.nikon.com/products/software/nis-elements/viewer

Arrange from low to high Selection choice Leica Nikon Olympus Zeiss No FL PLAN (achromatic) HI-PLAN (achromatic) FL only Visible N-PLAN (achromatic) FL PLAN (achromatic) CFI Achromat Series Achromat (ACH) Achromat FL-only visible CFI E Plan Achromat Series Planachromat (cell 2 culture) FL both UV +visible CFI Super Fluor Series Neo-flouor FL both UV +visible Semi-Apochromat - PL FLUOTAR (PL-FL) CFI Plan Fluor Series Semiapochromat (FL) Plan-Neofluor FL both UV +visible PL APO (apochromatic) CFI Apochromat Lambda S - Apochromat (APO) - Super Apochromat (SAPO) - A-line - Extended Apochromat (XAPO) - X-line Apochromatic FL both UV +visible CFI Plan Apochromat Lambda D Series Planapochromat https://www.thorlabs.com/