Annual Research Report 2023

Annual Research Report 2023

Faculty of Medicine, Ramathibodi Hospital


 

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Upright Fluorescence Microscope (update 18-Nov-2024)

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Model: Axio Imager 2 (Fully Motorized) - has started the mission on 26 September 2024 White light source: LED Illuminator microLED 2 1.7.1 423053-9072-000 Fluorescence light source: LED Colibri 7 , Type R[G/Y]B-UV - 423052-9730-000 UV (385/30 nm - 370-400 nm)   for excitation of DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue and similar dyes Blue (469/38 nm - 450-488 nm)   for excitation of FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC/ Fluorescein, Kaede (green /red), PerCP, YoYo-1, FuraRed and similar dyes Green (555/30 nm - 540-570 nm)   for excitation of TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange and similar dyes Yellow (590/27 nm - 576-603 nm)   for excitation of MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568,
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Quick links for all tools

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Tools 1.Inverted fluorescence microscope https://minicore-rarc.blogspot.com/2018/05/inverted-fluorescence-microscope.html 2.Upright fluorescence microscope https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html 3.UV-Vis spectrophotometer https://minicore-rarc.blogspot.com/2019/02/genesys-10s-uv-vis-spectrophotometer.html 4.UVP Hybridization https://minicore-rarc.blogspot.com/2020/02/uvp-hybridization.html 5.Boekel Hybridization https://minicore-rarc.blogspot.com/2020/02/wi-mi-09boekel-big-shot-iii.html 6.Electroporation https://minicore-rarc.blogspot.com/2020/10/electroporation.html Online calendar for all instruments that I have been assigned as custodian. Click How to book the instrument Guidelines to determine the authorship for the people who work in the core facility Click RC Instrument list (yearly check)
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